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Application Notes
This application note describes how PIPETMAX® with qPCR Assistant is a highly proficient pipetting workstation that reduces the potential for cross-contamination and other human induced errors.
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
The Bradford Assay is a common universal protein concentration determination technique. In this app note, you'll learn how the automation of this qualitative application shows comparability between manual and automated results for preparing a standard curve and samples for quantification of protein in solution.
Reagents used in qPCR master mixes, including buffers, surfactants, and detergents, can negatively influence accuracy and precision, thereby increasing sample variability and decreasing data reliability. In this app note, we used PIPETMAX® to test whether the number of times a tip was pre-wet before transferring master mix affected qPCR performance.
Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.
Gene expression studies are often used to gain insight into complex interactions between organisms, including those between plants and viruses. Real-time PCR (qPCR) is a tool frequently used in these studies given its wide dynamic range, sensitivity, and ease of automation. Learn how PIPETMAX can be used to automate qPCR sample prep in this informative app note.
Solid phase reversible immobilization (SPRI) beads are commonly used to clean up polymerase chain reaction (PCR) and DNA samples for downstream applications, such as sequencing and cloning. This application note demonstrates a flexible and reliable automated procedure for next-generation sequencing (NGS) library cleanup.
This application note describes the setup and execution of automation of the Illumina® Nextera® XT DNA Library Preparation Kit using the PIPETMAX automated liquid handler. Five different scripts have been created and are described in detail in this document.
Automation of the Kinase Selectivity Profiling System with PIPETMAX, in conjunction with automated detection and data analysis methods, offers a streamlined workflow for single-point and dose-response evaluation of potential cancer therapies. Learn more in this application note.
Quantitative real time PCR, often coupled with reverse transcription, is a very sensitive technique that can be used for many applications including gene expression analysis. PIPETMAX Normalization Assistant delivers an easy-to-use software and hardware solution to automate normalization of sample concentration without compromising RNA integrity.