Gilson Connect
This application note describes the setup and execution of automation of the Illumina® Nextera® XT DNA Library Preparation Kit using the PIPETMAX 268 automated liquid handler. Five different scripts have been created and are described in detail in this document.
Gene expression studies are often used to gain insight into complex interactions between organisms, including those between plants and viruses. Real-time PCR (qPCR) is a tool frequently used in these studies given its wide dynamic range, sensitivity, and ease of automation. Learn how PIPETMAX 268 can be used to automate qPCR sample prep in this informative app note.
Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX 268 automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.
Reagents used in qPCR master mixes, including buffers, surfactants, and detergents, can negatively influence accuracy and precision, thereby increasing sample variability and decreasing data reliability. In this app note, we used PIPETMAX® 268 to test whether the number of times a tip was pre-wet before transferring master mix affected qPCR performance.
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX 268. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has been used to create useful animal models for studying complex diseases, as well as correcting or inducing disease states within a cell. This technical note describes how PLATEMASTER and EXTRACTMAN can be used in the rapid enrichment and clonal selection of CRISPR modified cells.
This application note presents a new approach for the cleanup of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN® with the convenience of a DNA purification using MACHEREY-NAGEL’s proven NucleoMag® technology.
RNA-binding proteins (RBPs) are involved in many key cellular processes, but identification of the native binding partners (target RNAs) can be challenging. In this application note, we show that EXTRACTMAN can be used to efficiently and specifically enrich endogenously expressed ribonucleoprotein complexes.
EXTRACTMAN® improved isolation speed nine-fold and produced equivalent or better yields of purified DNA compared to tube-based isolation methods. In this application note, four DNA samples from two different organisms (mammalian and plant) were processed in parallel with no cross-contamination.
This application note describes how PIPETMAX® 268 with qPCR Assistant is a highly proficient pipetting workstation that reduces the potential for cross-contamination and other human induced errors.
